mouse anti dsdna igg elisa kit  (Alpha Diagnostics)

Journal: PLOS One

Article Title: GBP2 promotes podocyte pyroptosis and contributes to the pathogenesis of pediatric lupus nephritis

doi: 10.1371/journal.pone.0344601

Figure Lengend Snippet: A Evaluation of urinary protein-creatinine ratio, kidney-to-body weight ratio, blood urea nitrogen and anti-dsDNA antibody levels in control and Lupus nephritis (LN) mice. B Representative immunohistochemical staining of renal tissues in control and LN mice (400 × magnification; scale bar, 50 μm). C Evaluation of pyroptosis-related mRNA levels by RT-qPCR. D Evaluation of pyroptosis-related protein levels by western blotting. Data were expressed as the mean ± SD (n = 6 per group). * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Following the manufacturer’s protocol, the concentrations of double-stranded DNA (dsDNA) (Cusabio Technology, Wuhan, China; CSB-E11194m) in mouse serum and the levels of IL-1β (ELK Biotechnology, Wuhan, China; ELK1271) and IL-18 (ELK Biotechnology; ELK2269) in the culture supernatant of MPC-5 cells were quantified using ELISA.

Techniques: Control, Immunohistochemical staining, Staining, Quantitative RT-PCR, Western Blot

Journal: Nature Communications

Article Title: Lactate bridges mesangial cells to the differentiation of follicular helper T cells in lupus nephritis

doi: 10.1038/s41467-025-67416-x

Figure Lengend Snippet: a , b Single-cell analysis of kidney samples from healthy controls and SLE patients showing T cell subset proportions ( n = 7 (HC) or 11 (SLE) independent biological samples). c Correlation between renal Tfh frequency and SELENA-SLEDAI Total Score ( n = 8 independent biological samples). d–f PBMCs from healthy doners or SLE patients were adoptively transferred into NSG for 4 weeks to develop humanized chimeras. d BCL6⁺ CD4⁺ T cell proportions ( n = 8 independent biological samples). e CXCR5 hi PD-1 hi CD4⁺ T cell proportions ( n = 8 independent biological samples). f Correlation between renal Tfh frequency and urine protein ( n = 16 independent biological samples). g–m Humanized LN chimeras were treated with AAV2-shNC/shBCL6 ( n = 9 independent biological samples). g Schematic representation (Created in BioRender. Wen, Z. ( https://BioRender.com/dir2po9 ). h Renal Tfh proportions. i Serum human IgG anti-dsDNA. j Renal IgG. White arrows (IgG). Scale bar: 100 μm. k HE staining. Black circle (Glomerulus). Scale bar: 100 μm. l Urine protein. m , n Kidney organoids were exposed to healthy or SLE plasma for 24 h with OKT-3 (1 μg/ml), then co-cultured with purified healthy CD4⁺ T cells for 4 days ( n = 9 independent biological samples). m Schematic representation (Created in BioRender. Wen, Z. ( https://BioRender.com/dir2po9 ). n The frequency of Tfh cells. o-r MCs exposed to healthy or SLE plasma for 24 h, followed by incubation with OKT-3 (1 μg/ml). Subsequently, purified CD4 + T cells were co-cultured with MCs at a ratio of 5:1 for 4 days. o The frequency of Tfh cells ( n = 12 independent biological samples). p IL-21 expression following PMA (50 ng/ml)/Brefeldin A (5 μg/ml)/Ionomycin (500 ng/ml) stimulation ( n = 7 independent biological samples) in CD4 + T cells. q BCL6 mRNA expression analysis ( n = 8 independent biological samples). r Immunoblot analysis of BCL6 protein expression ( n = 12 independent biological samples). The data are represented as the mean ± SEM. Normality and lognormality are assessed using the Shapiro-Wilk test. Two-tailed unpaired t test ( b , e ), two-tailed Mann Whitney test ( d ), two-tailed paired t test ( h , i , n–q ), two-tailed Wilcoxon matched-pairs signed rank test ( l , r ), a simple linear regression analysis ( c , f ). Source data are provided as a Source Data file.

Article Snippet: IgG anti-dsDNA levels were measured with Human anti-dsDNA IgG ELISA Kit (CUSABIO, CSB-E04911h) and Mouse anti-dsDNA IgG ELISA Kit (Chondrex, 3031).

Techniques: Single-cell Analysis, Staining, Clinical Proteomics, Cell Culture, Purification, Incubation, Expressing, Western Blot, Two Tailed Test, MANN-WHITNEY

Journal: Nature Communications

Article Title: Lactate bridges mesangial cells to the differentiation of follicular helper T cells in lupus nephritis

doi: 10.1038/s41467-025-67416-x

Figure Lengend Snippet: a Renal lactate levels in humanized chimeras ( n = 20 independent biological samples). b Glucose metabolism pathways in MCs from single-cell data (adjusted p value via “clusterProfiler” GSEA analysis). c-g MCs were stimulated with healthy or SLE plasma ( n = 4 ( c ), 3 ( f ), 12 ( g , left) or 6 ( g , right) independent biological samples). h–j Healthy CD4⁺ T cells were co-cultured with LN-associated MCs pre-treated with GSK2837808A (60 μM) ( n = 10 ( h ) or 8 ( i ) independent biological samples). k , l Healthy CD4⁺ T cells were co-cultured with LN-associated MCs transfected with shLDHA or shNC ( n = 10 independent biological samples). m Heatmap of differentially expressed glucose metabolism genes in CD4 + T cells co-cultured with MCs ( n = 4 independent biological samples). n Intracellular lactate content in CD4 + T cells co-cultured with MCs ( n = 6 independent biological samples). o Healthy CD4⁺ T cells were co-cultured with D-Glucose-¹³C 6 -labeled MCs for 2 days, and ¹³C-labeled lactate in CD4⁺ T cells was measured by mass spectrometry ( n = 5 independent biological samples). p–r Healthy CD4 + T cells were co-cultured with LN-associated MCs transfected with shMCT4 or shNC ( n = 6 independent biological samples). s–u Healthy CD4⁺ T cells were transduced with shMCT1 or shNC and co-cultured with LN-associated MCs ( n = 6 independent biological samples). v Mice were intraperitoneally injected with 4-Hydroxytamoxifen (100 mg/kg), immunized with immunogenic self-DNA (50 μg/mouse), and detected for the renal Tfh cells, circulating IgG anti-dsDNA and urine protein after 8 weeks ( n = 7 independent biological samples). w–y Anti-CD3/28 bead-activated healthy CD4 + T cells were stimulated with L-lactic acid (L-lac, 10 mM) or sodium L-lactate (LacNa, 40 mM) for 4 days ( n = 7 independent biological samples). The data are represented as the mean ± SEM. Normality and lognormality are assessed using the Shapiro-Wilk test. Two-tailed unpaired t test ( e , f , c , v ), two-tailed Mann-Whitney test ( a , g , o ), two-tailed paired t test ( h , i , m , o–q , s , t ), paired RM one-way ANOVA ( k , n , w–y ). Source data are provided as a Source Data file.

Article Snippet: IgG anti-dsDNA levels were measured with Human anti-dsDNA IgG ELISA Kit (CUSABIO, CSB-E04911h) and Mouse anti-dsDNA IgG ELISA Kit (Chondrex, 3031).

Techniques: Clinical Proteomics, Cell Culture, Transfection, Labeling, Mass Spectrometry, Transduction, Injection, Two Tailed Test, MANN-WHITNEY

Journal: Nature Communications

Article Title: Lactate bridges mesangial cells to the differentiation of follicular helper T cells in lupus nephritis

doi: 10.1038/s41467-025-67416-x

Figure Lengend Snippet: a–e PBMCs from SLE patients were adoptively transferred into NSG to develop humanized LN chimeras, followed by treatment with or without sodium oxamate (OXA, 500 mg/kg) every 3 days until 28 days ( n = 6 ( a , b ) or 9 ( e ) independent biological samples). a The proportion of Tfh cells in the kidneys. b Serum human IgG anti-dsDNA. c Renal deposition of human IgG. White arrows (IgG). Scale bar: 100 μm. d HE staining. Black circle (Glomerulus). Scale bar: 100 μm. e Urine protein. f-j PBMCs from healthy donors were cultured with or without L-lac (10 mM) for 2 days. Stimulated PBMCs were injected into NSG mice to develop humanized chimeras ( n = 6 ( f , g ) or 8 ( j ) independent biological samples). f The proportion of Tfh cells in the kidneys. g Serum human IgG anti-dsDNA. h Renal deposition of human IgG. White arrows (IgG). Scale bar: 100 μm. i HE staining. Black circle (Glomerulus). Scale bar: 100 μm. j Urine protein. k–o CD4 + T cells isolated from PBMCs of SLE patients were activated with anti-CD3/28 beads and transduced with shPCAF or shNC. After 24 h, PCAF-kd CD4 + T cells mixed with other PBMC components were adoptively transferred into NSG to develop humanized LN chimeras ( n = 4 independent biological samples). k The proportion of Tfh cells in the kidneys. l Serum human IgG anti-dsDNA. m Renal deposition of human IgG. White arrows (IgG). Scale bar: 100 μm. n HE staining. Black circle (Glomerulus). Scale bar: 100 μm. o Urine protein. The data are represented as the mean ± SEM. Normality and lognormality are assessed using the Shapiro-Wilk test. Two-tailed paired t test ( a , e , f , g , j , k , o ), two-tailed Wilcoxon matched-pairs signed rank test ( b , l ). Source data are provided as a Source Data file.

Article Snippet: IgG anti-dsDNA levels were measured with Human anti-dsDNA IgG ELISA Kit (CUSABIO, CSB-E04911h) and Mouse anti-dsDNA IgG ELISA Kit (Chondrex, 3031).

Techniques: Staining, Cell Culture, Injection, Isolation, Transduction, Two Tailed Test